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KMID : 0360220110520070852
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Journal of the Korean Ophthalmological Society
2011 Volume.52 No. 7 p.852 ~ p.858
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Effects of Calf Serum on Human Corneal Epithelial Cells in Vitro
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Lee Jong-Soo
Kim Eun-Hee
Kim Su-Jin
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Abstract
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Purpose:To investigate the biologic effects of topical calf serum on corneal epithelial cells in vitro.
Methods:The effects of calf serum on the corneal epithelial cells were evaluated using the MTT (3-[4,5-Dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide) assay, and the concentration of IL-1α, TGF-β1 and MMP-9 in the cells was measured. Cell damage was determined using lactate dehydrogenase (LDH), and cellular morphologies were examined by transmission electromicroscopy.
Results:Metabolic activity of the corneal epithelial cells decreased at higher concentrations and longer exposure durations. IL-1α, TGF-β1 and MMP-9 titers were lower in calf serum-treated cells than in the control. LDH and cellular damage to the corneal epithelial cells, such as chromatin margination and cytoplasmic organelle swelling, were prominent in cells treated with 30% calf serum.
Conclusions:Cellular metabolic activity was higher and cellular toxicity was lower in cells treated with 10% calf serum compared to those treated with the 20% and 30% concentrations. Furthermore, inflammatory cytokines were sufficiently inhibited in cells treated with the 10% solution. These results indicate that 10% calf serum could be used clinically.
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KEYWORD
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Calf serum, Corneal epithelial cell toxicity, LDH, MTT
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